nf-κb activation hcs hitkit Search Results


nf κb hitkit  (Thermo Fisher)
99
Thermo Fisher nf κb hitkit
MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor <t>TNF-α-induced</t> <t>NF-κB</t> nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
Nf κb Hitkit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nf-%CE%BAb+activation+hcs+hitkit/pmc00224571-63-32-34?v=Thermo+Fisher
Average 99 stars, based on 1 article reviews
nf κb hitkit - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier

Image Search Results


MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Journal:

Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

doi: 10.1128/JVI.77.18.10125-10130.2003

Figure Lengend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

Article Snippet: BM macrophages were mock or MCMV infected for 18 h and then stimulated with TNF-α (10 ng/ml) for 30 min. Macrophages were then fixed, washed, and stained for NF-κB by using the NF-κB HitKit (Cellomics) per the manufacturer's directions, yielding green color (fluorescein isothiocyanate [FITC]).

Techniques: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection